The generic position of ustilago maydis, ustilago scitaminea, and ustilago esculenta ustilaginales. An artificial inoculation method was used by immersing 1 bud cutting in a spores. Intraspecies diversity within ustilago scitaminea isolates from south africa, reunion island, hawaii and guadeloupe was assessed by rapds, b e matingtype gene detection, rdna sequence analysis, microscopy and germination and morphological studies. Evidence for the dispersal of a unique lineage from asia to america and africa in the sugarcane fungal pathogen ustilago scitaminea. The disease then appeared in argentina in 1940, followed by reports from hawaii, fiji, dominican. According to the nuclear behaviour, the mycelium of ustilago passes. Pseudozyma, one recently isolated strain, ustilago scitaminea, has been shown to exhibit abundant melb production from sugarcane juice. Efforts made so far to check its destructive effects on the growing sugarcane industry have centred on. A brown cerebriform colony was usually the first form to grow from tissue. Smut is a major disease of sugarcane plants and is caused by the fungus ustilago scitaminea. Ustilago, the largest genus of the family ustilaginaceae is represented by more than 400 cosmopolitan species. Pdf some biological parameters of the sugarcane smut fungus. Mctaggart et al ustilago sporisoriummacalpinomyces complex 57 in sorosporium and ustilago have since been reclassi. In this article we will discuss about the life cycle of ustilago with the help of suitable diagrams.
Isolation and identification of differentially expressed genes in sugarcane infected by ustilago scitaminea. Cp 881165 was planted in stage 2 at canal point in november. Ustilago scitaminea infects sugarcane plants through buds. Pdf pathogenic variability of ustilago scitaminea from. Pdf survival of teliospores of sugarcane smut caused by ustilago. Differential gene expression in sugarcane in response to challenge by fungal pathogen ustilago scitaminea revealed by cdnaaflp. It has since been reported in most of the sugarcanegrowing countries in asia and africa in the eastern hemisphere 1.
Isolation and identification of differentially expressed. Evidence for the dispersal of a unique lineage from asia. Proc s afr sug technol ass 2004 78 isolation and characterisation of some endophytic bacteria from papua new guinea sugarcane j omarjee1, t van antwerpen1, j. Molecular profiling demonstrates limited diversity amongst. Even though sugarcane smut is worldwide in distribution, the existence of different s. Intraspecies diversity within ustilago scitaminea isolates from south africa, reunion island, hawaii and guadeloupe was assessed by rapds, b e matingtype gene detection. The efficacy of nine fungicides with different concentrations was tested against ustilago scitaminea, using spore germination inhibition technique.
It has since been reported in most of the sugarcanegrowing countries in asia and africa in the eastern. Both methods confirm the recently published nomenclatural change of the cane smut ustilago scitaminea to sporisorium scitamineum and indicate a putative connection between ustilago maydis. Differential gene expression in sugarcane during sugarcane ustilago scitaminea interaction was conducted in a smutresistant genotype. The sugarcane smut fungus, ustilago scitaminea, first appeared in australia in the ord river irrigation area oria in july 1998. Growth and sporulation of ustilago scitaminea, in vivo and in vitro. Pdf molecular profiling demonstrates limited diversity. Sugarcane smut ustilago scitaminea is the most prevalent and serious disease of sugarcane in nigeria. The fungus was easily isolated from sugarcane tissue and cultured on six different media. Efforts made so far to check its destructive effects on the growing sugarcane. Two hyphal forms of ustilago scitaminea were observed in vivo. Relationships between phenolicsconjugated polyamines and. Download fulltext pdf download fulltext pdf pathogenic variability of ustilago scitaminea from sao paulo state article pdf available in summa phytopathologica 352.
Effect of carbon sources on germination of chlamydospores of ustilago scitaminea syd. Only spores germinating on immature tissues at the base of inner bud scales readily formed. For full access to this pdf, sign in to an existing account, or. Sugarcane smut is a fungal disease of sugarcane caused by the fungus sporisorium scitamineum.
Twelve populations of the pathogen were collected from three sugarcane. Production of glycolipid biosurfactants, mannosylerythritol lipids, by a smut fungus, ustilago scitaminea nbrc 32730 tomotake morita,1 yuko ishibashi,2 tokuma fukuoka,1 tomohiro imura,1 hideki. Ustilago scitaminea nbrc 32730 has been reported mainly to produce a monoacetylated and diacylated mel, melb, from sucrose as sole carbon source. Sydow, and leaf scald caused by xanthomonas albilineans ashby dowson by natural infection. Citeseerx document details isaac councill, lee giles, pradeep teregowda. Ustilago scitaminea 25,33, occurs worldwide and is one of the most prevalent fungal diseases of sugarcane in china. This study was conducted to estimate the degree of association of smut ustilago scitaminea syd. Impact of pathogen genetics on breeding for resistance to sugarcane diseases by philippe c. Butler and bisby 1958 reported 108 species from india.
Pdf reaction of twenty sugarcane clones to smut disease. Survival of teliospores of sugarcane smut caused by ustilago scitaminea in various conditions of south east queensland. Molecular phylogeny of ustilago and sporisorium species. In vitro detection of yeastlike and mycelial colonies of.
Association and heritability of sugarcane smut resistance. Pdf sugarcane smut, caused by ustilago scitaminea, is an important disease of sugarcane. Barbados 42231 smut is a major disease of sugarcane caused by ustilago with teliospores of ustilago scitaminea changes the scitaminea. Relationships between phenolicsconjugated polyamines and sensitivity of sugarcane to smut ustilago scitaminea. The disease is known as culmicolous, which describes the outgrowth of fungus of the. More precisely, sporisorium contains at least 60 taxa originally classi.
Pathogenic variability of ustilago scitaminea from. Isolation and identification of yeastlike and mycelial colonies of ustilago scitaminea using specific primers. The most likely source of this infection was thought to be wind blown spores. Except for sequence data, the other analyses yielded no differences in the isolates that could be used in a phylogenetic separation. Efforts made so far to check its destructive effects on the growing sugarcane industry have centred on use of singlestrategy controls. Barbados 42231 with teliospores of ustilago scitaminea changes the pattern of polyamine conjugation in several org.
Growth and sporulation of ustilago scitaminea, in vivo and. Sugarcane plants of the cultivar b72 191 have been cultivated in tubes and inoculated with a pure culture of smut fungus ustilago scitaminea sydow in an otherwise microorganismfree environment. Pdf the survival of sugarcane smut teliospores in the field is an important factor in the epidemiology of the. Pdf survival of teliospores of sugarcane smut caused by. The generic position of ustilago maydis, ustilago scitaminea, and. Survival of teliospores of ustilago scitaminea under various field conditions in southeast queensland. Management of sugarcane smut ustilago scitaminea with. Structural analyses of these compounds suggest a role for mels.
Smut of sugarcane is caused by the fungus ustilago scitmainea sydow, 1924. Differential gene expression in sugarcane in response to. Pdf control of sugarcane smut ustilago scitaminea syd. Using cdnaaflp along with silver staining, a total of 6 transcriptderived fragments tdfs were found to be differentially expressed in response to challenge by u. Penyakit luka api tebu pertama kali ditemukan di afrika selatan pada tahun 1877 dan penyebaran luka api meliputi sebagian besar daerah penghasil tebu yaitu indonesia nzioki. Isolation and characterisation of some endophytic bacteria. Molecular profiling demonstrates limited diversity amongst geographically separate strains of ustilago scitaminea. Inoculation of in vitro cultivated sugarcane with smut. Amplified fragment length polymorphism aflp analysis of cdna was used to identify sugarcane genes differentially expressed in diseaseresistant but not in susceptible sugarcane somaclones in response to inoculation with either ustilago scitaminea. Effect of carbon sources on germination of chlamydospores. Ustilago scitaminea florida department of agriculture.
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